Fluorescence lifetimes for TPP in the indicated membranes measured with the time correlated single photon counting (TCSPC) technique; excitation at 465 nm and emission collection at 650 nm and 720 nm (averaged lifetimes from the two emission wavelengths; in brackets: fractional intensities). FLIM fluorescence lifetimes with fractional intensity of the fitted sample area, excitation at 640 nm and emission centred at 715 nm.
| Sample | τ TCSPC (ns) (Ii%) | τ FLIM (ns) (Ii%) |
|---|---|---|
| CA_0.05% TPP | 0.7 (28%); 10.2 (72%) | 1.8 (13%); 8.1 (87%) |
| CA_0.1% TPP | 0.4 (23%); 10.5 (77%) | 1.9 (14%); 8.3 (86%) |
| CA_0.5% TPP | 0.4 (16%); 11.0 (84%) | 1.0 (2%); 10.4 (98%) |
| CA_1.0% TPP | 0.3 (34%); 10.9 (66%) | 0.5 (29%); 3.1 (11%); 10.0 (60%) |
| CA_SURF_0.1% TPP | 1.0 (53%); 9.0 (47%) | 0.7 (32%); 2.6 (13%); 9.0 (55%) |