Fluorescence lifetimes for TPP in the indicated membranes measured with the time correlated single photon counting (TCSPC) technique; excitation at 465 nm and emission collection at 650 nm and 720 nm (averaged lifetimes from the two emission wavelengths; in brackets: fractional intensities). FLIM fluorescence lifetimes with fractional intensity of the fitted sample area, excitation at 405 nm and emission centred at 655 nm.
Sample | τ TCSPC (ns) (Ii%) | τ FLIM (ns) (Ii%) |
---|---|---|
CA_0.01% GO_0.1% TPP | ≤0.1 (12%); 11.5 (88%) | 0.5 (1%); 10.7 (99%) |
CA_SURF_0.01% GO_0.1% TPP | 0.4 (27%); 9.0 (73%) | 0.4 (16%); 2.3 (15%); 9.1 (69%) |