Skip to main content
. Author manuscript; available in PMC: 2023 Sep 7.
Published in final edited form as: Inflamm Res. 2015 Nov 25;65(1):71–80. doi: 10.1007/s00011-015-0892-z

Figure 2. Rat tracheal epithelial cells express Duox and produce extracellular H2O2 in a calcium-dependent manner.

Figure 2.

Air-liquid interface cultures of primary rat tracheal cells were cultured for 17 days and subjected to the following analyses: (A) Duox protein was detected in cell lysates by western blotting. Samples of three animals shown. (B) Gene expression levels of rat duox1, duox2 and actin genes were detected by reverse transcriptase PCR. Samples of three animals shown. (C) Kinetics (left) and endpoint (right) measurements of extracellular hydrogen peroxide production detected by homovanillic acid oxidation assay. Kinetics: one representative result, n=3. ATP (100 μM), ionomycin (1 μM). Endpoint: mean+/−S.E.M., n=3. Extracellular calcium was chelated by addition of 1mM EGTA. IB, immunoblot; RT, reverse transcriptase; RFU, relative fluorescence unit.