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. 2023 Aug 24;16:1183315. doi: 10.3389/fnmol.2023.1183315

Figure 4.

Figure 4

MACROH2A1 and MAP4 RNA are detected in axons with axotomy. (A) Cortical neurons in microfluidic chambers without axotomy have little detectable MACROH2A1 (Red) and MAP4 (Green) by RNAscope in growing axons immunostained with β-III tubulin (Blue). After Axotomy both multiple transcripts of MACROH2A1 (red arrow) and MAP4 (green arrow), several hundred microns further away when comparing to No Axotomy. We preformed 4 independent experiments. (C) Quantification of MAP4 RNA in 100 micron increments within the microgroove with and without axotomy. The graph is the average of 4 independent experiments. (D) Quantification of MACROH2A1 RNA in 100 micron increments within the microgroove with and without axotomy. The statistics is t-test with *p < 0.05 and **p < 0.01. The neurons are also immunostained with β-III tubulin which is shown in blue.