Figure 5.

(A) MACROH2A1 and MAP4 RNA are detected in the white matter of rat spinal cord. Both MACROH2A1 and MAP4 transcripts are detected by RNAscope in the white matter of the uninjured rat spinal cord in the transverse plane, cell nuclei are stained with DAPI (blue). Looking at the magnified region in the white box, both MACROH2A1 and MAP4 transcripts are detected outside of DAPI positive nuclei and in the white matter. (B) Sagittal section of spinal cord revealing axons are immunostained with β-III tubulin (Blue) and RNAscope detection of MACROH2A1 (Red), revealing the presence of this RNA transcript along the axons.