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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Mitochondrion. 2023 Mar 15;70:31–40. doi: 10.1016/j.mito.2023.03.003

Figure 5. Overexpression of miR-499-5p neutralizes the gp120 effect.

Figure 5.

(A) Relative expression of miR-499-5p normalized to the untreated control (log10) indicating the overexpression of miR-499-5p using a mimic-499-5p and U6 as an internal loading control. (B) Overexpression of miR-499-5p led to inhibition in Calcineurin expression, thus rescuing the effect of gp120. (C) Relative DRP1 expression level normalized to internal control. The GAPDH represents the effect of overexpressed miR-499-5p that caused the inhibition of calcineurin and decreased expression of Drp1. Data represent the mean ± S.D. Results are statistically significant (p<0.05 by analysis of variance, *p<0.05; **p<0.01; ***p<0.001). (D) Cells transfected with miR-499-5p displayed elongated mitochondrial structure and rescued the effect of gp120. (Control=Ctrl)