FIGURE 3.

Diversity of the DARPin sequence sub-library. Unrooted phylogenetic tree from hierarchical clustering of sequences by the chemical properties of amino acids using defined amino-acid homology groups (see Methods section). Sequences marked in black are from the mutation-based set and in blue from the permutation-based set. The two positive-control sequences are shown in red. Only a 5% random sample of the sub-library consisting of 134 sequences is plotted. The top-18 consensus designs and positive controls were annotated. (A) For visual clarity, the terminal branches were equally trimmed down to a cladogram giving the illusion of sequence proximity (Yu, 2020). (B) The non-trimmed tree that preserves the ordering in (A) is shown to illustrate the true divergence in sequence between designs. For reference, the evolutionary distance is shown.