Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 24;51(16):8850–8863. doi: 10.1093/nar/gkad618

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 4. — Isothermal calorimetry of PCBP2 domains with poly-rC containing RNAs. (A) Interaction of PCBP2 KH1/2 domain with PV cloverleaf RNA. Isolated stem–loop B, WT cloverleaf (tRNA-CL^PV) and A39U mutant cloverleaf (tRNA-CL^A39U) were titrated into PCBP2 KH1/2 domain. Thermograms and fitted binding curves are shown. Data were fit with single binding-site model. Titrations were repeated at least twice. (B) Interaction of PCBP2 KH3 domain with PV cloverleaf RNA. Isolated stem–loop B, WT cloverleaf and A39U mutant cloverleaf were titrated into PCBP2 KH3 domain. The data were fit with single binding-site model. Note KH3 domain binds A39U mutant cloverleaf with higher affinity than WT cloverleaf, suggesting that stem–loop B site is not accessible in WT cloverleaf. (C) Interaction of PCBP2 domains with PV 3′poly-rC site. The linear ssRNA containing the 3′poly-rC sequence was titrated into either KH1/2 or KH3 domains. The binding affinities to the linear ssRNA are similar for both PCBP2 domains. Note that the KH3 domain binds 3′poly-rC with 2:1 (protein/RNA) stoichiometry. Summary of parameters are listed in Table S1.