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. 2023 Jul 14;51(16):8820–8835. doi: 10.1093/nar/gkad568

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Slf1 is required for the eIF4G1-mediated response to oxidative stress. (A) Slf1-TAP was immunoprecipitated from an exponential phase whole cell extract either unstressed or treated with 0.4 mM H₂O₂ for 15 minutes, formaldehyde, or RNase I. The bound (IP) fractions were analysed by Western blotting and membranes probed with antibodies for Slf1, eIF4G1 and eIF4G2. (B) The wild-type (BY4742), homogenic strains (4G1h, 4G2h), and strains deleted for SLF1 (slf1, 4G1h slf1, 4G2h slf1) were grown to exponential phase and the A₆₀₀ adjusted to 1, 0.1, 0.01 or 0.001 before spotting onto the indicated plates. (C) Volcano plot of the log₂ fold change and –log₁₀P value of the pairwise comparisons between the homogenic strains in oxidative stress conditions with Slf1 mRNA targets highlighted in purple. Box and whisker plots show the average fold changes for all proteins and Slf1 mRNA targets.