Skip to main content
. 2023 Sep 4;55(9):1531–1541. doi: 10.1038/s41588-023-01480-1

Extended Data Fig. 10. Analysis of Trp53-mutant mice following inflammatory challenge.

Extended Data Fig. 10

a, IFNγ level in spleen serum 4 h after poly(I:C) injection. n = 6 mice per group from 2 independent experiments. Lines indicate mean ± s.e.m. and “p”, two-tailed unpaired t-test p-value. b-c, Gating strategy for mouse chimaera experiments (Related to Fig. 5) used to quantify BM LSK and HSCs populations (b) and myeloid cells in the peripheral blood (PB) (c). d-g, Analysis of WT:Trp53R172H/+ chimaera mice treated with 3 cycles of 6 poly(I:C) injections (related to model setting 1, Fig. 5a) with serial readouts of CD45.1 Trp53R172H/+ Mac1+ PB cells (d), percentage of CD45.1 Trp53R172H/+ BM LSK (Lin−Sca-1+c-Kit+) (e), number of CD45.1 Trp53R172H/+ BM LSK (f) and CD45.2 WT BM LSK per million BM cells (g) 20 weeks post transplantation. n = 11-12 mice per group from 3 biological replicates in 2 independent experiments. h-k, Analysis of WT:Trp53R172H/+ chimaera mice treated with 3 cycles of 6 poly(I:C) injections (related to model setting 2, Fig. 5h) with serial readouts of white blood cells (h), hemoglobin (i) and platelet (j) counts measured every 2 weeks, and percentage of CD45.2 Trp53R172H/+ granulomonocytic (Ly6G and/or Mac1 + ) PB cells (k). l, Gating strategy for granulomonocytic (neutrophils and monocytes) and lymphoid (T, NK and B cells) populations in WT:Trp53R172H/+ chimaera mice. m, Percentage of CD45.2 Trp53R172H/+ BM HSC and LSK at 18 weeks post transplantation. n, Gating strategy for CFUE and PreCFUE populations in WT:Trp53R172H/+ chimaera mice. n = 22 control, n = 23 poly(I:C) groups (h-k) or n = 13 control, n = 14 poly(I:C) groups (m) from 2 independent experiments. Bars indicate mean ± s.e.m. and “p”, two-tailed unpaired t-test p-value.