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. 2023 Sep 4;55(9):1531–1541. doi: 10.1038/s41588-023-01480-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Experimental design of Vav-iCre WT:Trp53^R172H/+ chimera serial poly(I:C) and LPS treatment. b–e, Analysis of chimera mice 20 weeks post-transplantation following three cycles of six poly(I:C) injections. Percentage of CD45.1 Trp53^R172H/+ Mac1⁺ cells in the PB (b) or BM HSCs (Lin^-Sca-1⁺c-Kit⁺CD150⁺CD48⁻; c), number of BM CD45.1 Trp53^R172H/+ HSC (d) and CD45.2 WT HSC (e) per million BM cells. n = 11–12 mice per group in two independent experiments and three biological replicates. Mean ± s.e.m. is shown and P indicates a two-tailed unpaired t-test P value. f,g, Analysis of chimera mice 20 weeks post-transplantation following three cycles of eight LPS injections. Percentage of CD45.1 Trp53^R172H/+ Mac1⁺ cells in the PB (f), or BM LSKs (Lin^-Sca-1+c-Kit+; g). n = 10–11 mice per group in two independent experiments and two biological replicates. Mean ± s.e.m. is shown and P indicates a two-tailed unpaired t-test P value. h, Experimental design of SCL-Cre-ER^T WT:Trp53^R172H/+ chimera serial poly(I:C) treatment. i,j, Absolute counts of CD45.1 WT or CD45.2 Trp53^R172H/+ granulo-monocytic (Ly6G⁺ and/or Mac1⁺; i) and lymphoid (B220⁺/NK1.1⁺/CD3⁺; j) PB cells at 17 weeks post-transplant. k, Percentage of CD45.1 WT or CD45.2 Trp53^R172H/+ erythroid progenitors (Lin^-Sca-1^-c-Kit⁺CD41⁻FcgRII/III⁻CD105⁺) in total BM MNC at 18 weeks post-transplant. n = 22 control, n = 23 poly(I:C) groups (i, j) or n = 13 control, n = 14 poly(I:C) groups (k) from two independent experiments. Bars indicate mean ± s.e.m. and P indicates two-tailed unpaired t-test P value. l,m, Analysis of cell cycle (l) and apoptosis (m) in BM LSK cells from chimeric mice 18 weeks post-transplantation following three cycles of six poly(I:C) injections as in h. n = 13 control, n = 17 poly(I:C) groups (l) or n = 13 control, n = 14 poly(I:C) groups (m) from two independent experiments, mean ± s.e.m. is shown and P indicates adjusted P value from one-way Anova (in l, the P value was calculated using G0/G1 cell cycle phase).