Table 3.
Basal gene expression in A-72 cells and a neoplastic primary tissue (Hemangiosarcoma). Data are expressed as: + all samples were positive;—all samples were negative; ± only some samples were positive; ND: not detected. ΔCq = Cq (target gene)—Cq (reference gene) S.D.: standard deviation
| Gene | A-72 | ΔCq + S.D | Neoplastic tissue (HE) | ΔCq + S.D |
|---|---|---|---|---|
| CD14 | ± | 13.8 ± 2.0 | + | 1.5 ± 1.1 |
| CD44 | + | 1.9 ± 0.9 | + | 0.9 ± 0.7 |
| CXCR4 | + | 8.2 ± 1.8 | + | 4.6 ± 1.7 |
| ERBB2 | + | 2.9 ± 1.3 | + | 5.8 ± 0.9 |
| IL6 | + | 10.8 ± 1.7 | + | 2.8 ± 1.9 |
| IL8 | + | 3.2 ± 3.3 | + | 0.3 ± 1.8 |
| IL10 | - | ND | + | 4.9 ± 2.0 |
| IL15 | ± | 15.7 ± 1.3 | + | 5.3 ± 2.2 |
| IL18 | - | ND | + | 2.8 ± 1.8 |
| NOS2 | + | 7.3 ± 1.5 | + | 2.7 ± 2.2 |
| LY96 | + | 2.8 ± 1.7 | + | 1.9 ± 1.4 |
| MYD88 | + | 5.1 ± 0.7 | + | 6.0 ± 0.8 |
| NFKB/p65 | + | 0.3 ± 1.4 | + | 0.9 ± 1.2 |
| PTEN | + | 11.8 ± 2.4 | - | ND |
| TP53 | + | 0.8 ± 1.3 | + | 1.2 ± 0.3 |
| RAD51 | + | 2.8 ± 0.4 | + | 5.2 ± 1.8 |
| STAT5 | + | 2.8 ± 2.5 | + | 1.5 ± 0.9 |
| TGFβ | + | 2.2 ± 2.1 | + | 0.6 ± 0.2 |
| TLR4 | ± | 13.8 ± 2.4 | + | 3.1 ± 1.1 |
| TLR5 | + | 8.4 ± 2.7 | + | 12.5 ± 1.6 |