Table 1.
A spectral band position indicated in UV–Vis spectra of fresh whole blood and with anticoagulant (normal and saturated with CO2 and O2) and isolated cytochrome c in ferric (Fe3+) and ferrous (Fe2+) forms.
| Soret band | Q band spectral splitting | |||
|---|---|---|---|---|
| Qv | Q0 | |||
| Fresh whole blood | ||||
| Saturated with 15 min O2 | 414.43 ± 0.04 | – | 543.00 ± 0.30 | 578.00 ± 0.13 |
| Saturated with 10 min O2 | 414.97 ± 0.04 | – | 543.00 ± 0.22 | 578.00 ± 0.10 |
| Saturated with 5 min O2 | 415.61 ± 0.05 | – | 543.42 ± 0.11 | 577.49 ± 0.07 |
| Normal (control) | 417.68 ± 0.05 | – | 542.97 ± 0.13 | 578.18 ± 0.07 |
| Saturated with 5 min CO2 | 417.05 ± 0.32 | 433.36 ± 0.13 | 546.53 ± 0.23 | 576.96 ± 0.18 |
| Saturated with 10 min CO2 | 416.79 ± 0.42 | 432.48 ± 0.11 | 547.76 ± 0.14 | 577.53 ± 0.12 |
| Saturated with 15 min CO2 | 416.51 ± 0.48 | 431.88 ± 0.09 | 551.32 ± 0.45 | 578.66 ± 0.39 |
| Whole blood with EDTA | ||||
| Saturated with 15 min O2 | 414.36 ± 0.03 | – | 542.72 ± 0.08 | 577.04 ± 0.05 |
| Saturated with 10 min O2 | 414.75 ± 0.03 | – | 542.99 ± 0.08 | 577.18 ± 0.05 |
| Saturated with 5 min O2 | 414.78 ± 0.03 | – | 543.00 ± 0.12 | 577.50 ± 0.06 |
| Normal (control) | 415.20 ± 0.03 | – | 542.59 ± 0.11 | 577.23 ± 0.05 |
| Saturated with 5 min CO2 | 416.54 ± 0.16 | 433.32 ± 0.09 | 543.00 ± 0.22 | 577.50 ± 0.16 |
| Saturated with 10 min CO2 | 414.25 ± 0.06 | 433.71 ± 0.13 | 544.89 ± 0.11 | 576.66 ± 0.07 |
| Saturated with 15 min CO2 | 412.74 ± 0.03 | – | 542.57 ± 0.09 | 576.51 ± 0.05 |
| Cytochrome c | ||||
| Ferrous form (Fe2+, reduced) | – | 415.29 ± 0.09 | 518.89 ± 0.21 | 549.35 ± 0.06 |
| Ferric form (Fe3+, oxidized) | 407.64 ± 0.09 | – | 529.40 ± 0.21 | 549.64 ± 0.25 |