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. 2023 Sep 7;14:5500. doi: 10.1038/s41467-023-40968-6

Fig. 7. Astrocyte Orai1 cKO mice show distinct changes in inhibitory synaptic transmission following LPS challenge.

Fig. 7

a Schematic of the experimental timeline. WT and Orai1 cKO mice were injected with LPS intraperitoneally and brains were removed for slice electrophysiology ~18-20 h later. b Schematic of the slice-patch clamp recordings carried out in panels C-H. Whole-cell recordings were carried out in CA1 pyramidal neurons to record excitatory and inhibitory postsynaptic currents. c, d Example traces of sIPSCs in the indicated conditions and genotypes. e–h Summary graphs of the amplitude and frequency of sIPSCs. The amplitude and frequency were reduced in LPS administered Orai1 cKO mice but not WT mice. Data are presented as mean values +/- SEM. (e, g). Cumulative distribution graphs showing amplitude and inter-event interval (IEI) for WT (f) and cKO (h) mice illustrate the effect of LPS on Orai1 cKO mice. The number of recordings were as follows: n = 9 WT cells from 3 male & 1 female mouse, n = 13 WT + LPS cells from 2 female & 3 male mice, n = 11 WT + LPS+Thr cells from 2 female & 3 male mice, n = 5 cKO cells from 4 male mice, n = 6 cKO+LPS cells from 2 male & 1 female mice, n = 6 cKO+LPS+Thr cells from 2 male & 1 female mice. Statistical tests were conducted by one-way ANOVA followed by Tukey posthoc tests for all comparisons. Illustrations created with BioRender.com. Source data are provided as a Source data file.