Table 2.
Effects of a single bout of exercise on SIRT1.
| Study | Participants | Exercise protocol | Sample | Biomarker | Technique | Post-exercise SIRT1 (vs rest) |
|---|---|---|---|---|---|---|
| Aird et al., 202125 | Recreationally active males (N = 9, 26.1 ± 5.1 years) | SIT (4 × 30 s all-out cycle sprints against resistance of 75% BM, at > 80% VO2max) on overnight fast | Muscle tissue | SIRT1 mRNA gene expression | Multiplex PCR | ↑ at 3 h |
| Cho et al., 202226 | Young men (MIA N = 10, HIA N = 10, 20.70 ± 1.34 years) | Treadmill MIA (65% VO2max) and HIA (85% VO2max) | Blood | SIRT1 enzyme levels | ELISA | ↑ in both conditions |
| Dumke et al., 200927 | Trained male cyclists (N = 40, 29.1 ± 2.4 years) | 3 h of cycling at ≈57% Wmax | Muscle tissue | SIRT1 mRNA gene expression | qPCR | ↑ at 0 h |
| Edgett et al., 201328 | Recreationally active males (N = 8 in each intensity, 21.9 ± 2.2 years) | HIIT (cycling at 11 × 60 s at 73% WRpeak, 8 × 60 s at 100% WRpeak, or 6 × 60 s at 133% WRpeak) | Muscle tissue | SIRT1 mRNA gene expression | RT-PCR | ↑ at 3 h in all conditions |
| Ghasemi et al., 202029 | Overweight women (trained N = 10, untrained N = 10, 23.58 ± 2.23 years) | Wingate test (4 × 30 s all-out cycling at .075 kg/kg BM) | Blood | Serum SIRT1 | ELISA |
↑ in trained No significant effect in untrained |
| Granata et al., 202030 | Males (trained N = 8, untrained N = 8, 20 ± 2 years) | HIIT (5 × 4 min cycling at ≈107.4% of ẆLT) | Muscle tissue | SIRT1 mRNA gene expression | qPCR | ↓ at 0 h in both conditions |
| Guerra et al., 201031 | Male P.E. students (N = 8, 23.4 ± .6 years) | 30 s Wingate test at 100 rpm, ≈120% VO2max on overnight fast | muscle tissue | SIRT1 protein content | Western blot | ↑ at 2 h |
| Margolis et al., 201732 | Physically fit men and women (cycling group N = 7, treadmill group N = 5, 22 ± 1 years) | 1.5 h of of cycling or loaded treadmill walk at ≈58% VO2peak on overnight fast | Muscle tissue | SIRT1 mRNA gene expression | RT-PCR | ↑ at 0 h and 3 h in both groups |
| Morales-Alamo et al., 2012 | Male P.E. students (N = 10, 25 ± 4 years) | 30 s Wingate test at 100 rpm, ≈120% VO2max on overnight fast | Muscle tissue | SIRT1 protein content | Western blot | No significant effect |
| Morales-Alamo et al., 201333 | Male P.E. students (N = 9, 25 ± 5 years) | 30 s Wingate test at 100 rpm, ≈120% VO2max on overnight fast | Muscle tissue | SIRT1 protein content | Western blot | No significant effect |
| Mendham et al., 201634 | Sedentary, obese, middle-aged men (N = 9 rugby, N = 9 cycling, 48.8 ± 1.7 years) | 40 min of touch rugby or cycling at RPE = 13–14 on overnight fast | Muscle tissue | SIRT1 protein content | Western blot | No significant effect in either condition |
| Potthast et al., 202035 | Recreational runners (N = 25, 27.2 ± 4.1 years) | GXT (16.7 W per minute) until voluntary exhaustion on bicycle | Blood | SIRT1 activity | Sirtuin activity assay | ↑ at 0 h |
| Radak et al., 201136 | Young sedentary (N = 6, 26.0 ± 4.5 years), young physically active (N = 6, 30.2 ± 7.9 years), old sedentary (N = 6, 63.4 ± 4.7 years), and old physically active (N = 6, 62.4 ± 2.9) | 45 min of treadmill run at 70–75% VO2max then increased to 90%VO2max and terminated at exhaustion, on overnight fast | Muscle tissue | SIRT1 mRNA gene expression | RT-PCR |
↑ at 0 h in young sedentary ↑ at 0 h in young active ↑ at 0 h in old sedentary No significant effect in old active |
| Skelly et al., 201737 | Sedentary participants (men N = 8, women N = 8, 22 ± 3 years) | SIT (3 × 20 s all-out cycling at ≈ 500W) | Muscle tissue | SIRT1 mRNA gene expression | RT-PCR |
↑ at 3 h in men ↑ at 3 h in women |
BM body mass, GXT graded exercise test, HIIT high-intensity interval training, HRmax maximum heart rate, RPE rating of perceived exertion using Borg scale, rpm revolutions per minute, SIT sprint interval training, W watts, Wmax maximal power, Wpeak peak power, WLT power at lactate threshold, WRpeak peak work rate, ↑ increase, ↓ decrease.