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. 2023 Sep 7;8:132. doi: 10.1038/s41541-023-00728-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Recombinant baculovirus (rBVs) rBac-H1m, rBac-N1m, rBac-H3m, rBac-N2m, and rBac-M1 were successfully identified using immunofluorescence assay (IFA) by HA, NA, and M1-specific antibody in Sf9 cells (×200). The scale bar indicates 20 μm. B The morphology of VLPs similar to natural influenza virions were successfully observed by transmission electron microscopy (TEM). The scale bar indicates 100 nm. C The VLPs could produce 2^10 HA titers, while no HA titer was observed in Mock control group. D Different concentrations of VLPs had relatively high neuraminidase activity, and the neuraminidase activity increased with the increase of VLPs concentration.