Fig. 5. Mosaic VLPs immunization elicits robust cross-reactive antibody responses in mice against N1- and N2-representative viruses.

BALB/c mice were vaccinated with VLPs and compared to QIV-vaccinated mice. The NI antibody response to N1 (A) or N2 (B) representative strains was determined by ELLA assay in mice immunized with boost immunization. A heatmap of NI titers was further constructed to better visualize cross-reactive antibody response for each vaccine. Data are presented as the mean with standard error (SEM) (n = 5; two-way ANOVA with Tukey multiple comparison), *P < 0.05, **P < 0.01.