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. 2023 Sep 7;13:14706. doi: 10.1038/s41598-023-41933-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The expression levels of P-AKT, P-p38, P-ERK1/2 and P-JNK were reduced by asarinin, and the effect of asarinin on the expression of P-AKT, P-p38, P-ERK1/2 and P-JNK were suppressed after PPARγ silencing. Western blotting (WB) detected the protein expression levels of P-AKT, AKT, p38, P-p38, ERK1/2, P-ERK1/2, JNK and P-JNK (A-C). After silencing the Pparg, WB was used to detect the protein expression levels of P-AKT, AKT, p38, P-p38, ERK1/2, P-ERK1/2, JNK and P-JNK (D-F). Data are expressed as mean ± standard deviation, and all experiments were repeated independently at least 3 times, sample size (n) = 3 for each group, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.