Fig. 1.

GlcNAcase production by Aeromonas caviae CHZ306 cultivated in the chitin-containing medium under different nitrogen supplementations (viz. M1–M12). a GlcNAcase activity at 12 h A. caviae CHZ306 cultivation. b Time-course profiles of GlcNAcase activity during A. caviae CHZ306 cultivation in the medium M9, M11, M13, M14, and M15. M1 (ammonium sulfate); M2 (ammonium acetate); M3 (ammonium nitrate); M4 (urea); M5 (casamino acids); M6 (meat extract); M7 (yeast extract); M8 (bacto peptone); M9 (peptone A); M10 (peptone G); M11 (corn steep liquor); M12 (tryptone); M13 (1:1 (w/w) corn steep liquor:peptone A); M14 (1:2 (w/w) corn steep liquor:peptone A); M15 (2:1 (w/w) corn steep liquor:peptone A). Corn steep liquor and peptone A combinations in different proportions (M13, M14, and M15) are depicted by gray curves and bars. c Bonferroni pairwise comparison correction of the GlcNAcase activities at 96 h obtained with different nitrogen sources. Each square represents the negative natural log of the p value of the t-test pairwise comparison between the GlcNAcase activity obtained using each nitrogen source. Since -ln(0.05) = 2.996, any value on this matrix above 3 will cross the statistical significance threshold