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. 2023 Sep 4;96:104788. doi: 10.1016/j.ebiom.2023.104788

Fig. 2.

Fig. 2

Quantification of circulating T follicular helper (cTfh) cells, cTfh1 and cTfh2 subset, and post/pre ratios of cTfh1 and cTfh2. PBMC were stained with fluorochrome-labeled mAbs and analyzed on a BD FACS Canto II flow cytometer. Quantification of A) cTfh (CD3+/CD4+/CD45RA-/CXCR5+) in 16 IBD patients (of these [n = 12] with anti-TNF α treatment and [n = 4] receiving integrin antagonists) and 10 controls (of these [n = 6] high responders and [n = 4] low responders before (pre) and one week after 2nd dose as % of total CD4+ T cells; B) cTfh1 and C) cTfh2 cells as % of total cTfh before (pre) and one week after 2nd dose; D) Ratios (% post 2nd/% pre vaccination) of cTfh1 and cTfh2 subsets shown in low-responder IBD patients with anti-TNF α treatment (n = 12) and low-responder controls (n = 4) as well as high-responders IBD patients treated with integrin antagonists (n = 4) and high-responder controls (n = 6), black line is arithmetic mean; and E) Ratios of cTfh1 and cTfh2 subsets combined in low-responders and high-responders of all groups, black line is arithmetic mean. ANOVA with linear contrasts; ∗p ≤ 0.05.