Fig. 3.

Quantification of activated cTfh1 and cTfh2 cells and correlation with S1-specific IgG Abs and S-specific B memory cells. A) Quantification of activated cTfh1 and cTfh2 (as % of total cTfh1 and cTfh2 cells) pre and post primary vaccination in 8 high-responders (squares) (of these [n = 3] IBD patients with integrin antagonists and [n = 5] high-responder controls) and 13 low-responders (circles) (of these [n = 9] IBD patients with anti-TNF α treatment and [n = 4] low responder controls), the shown percentages are [% activation post 2nd dose] minus [% activation pre vaccination], black line is arithmetic mean. Correlation of activated cTfh1 cells present after primary vaccination with B) hu-1 S1-specific IgG Ab levels after primary vaccination and C) after booster vaccination; with D) percentages of S-specific B memory cells after primary vaccination and E) after booster vaccination. Spearman rank correlation coefficients (r_s) with p-values obtained by bootstrapping; ∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01, ∗p ≤ 0.05.