Fig. 2.

CircXRN2 inhibits the proliferation and migration of bladder cancer cells in vitro and in vivo. a A CCK-8 assay was conducted to evaluate cell viability in control and circXRN2-overexpressing cells. b CircXRN2 suppressed the colony-formation ability of bladder cancer cells. c CircXRN2-induced cell apoptosis in T24 and EJ cells. d, e Transwell migration assays and wound healing assays were conducted to evaluate cell migratory abilities. f Schematic illustration of the subcutaneous tumorigenesis model in nude mice with negative control or circXRN2-overexpressing cells. g-i CircXRN2 inhibited tumor growth of human bladder cancer in vivo (n = 5). j Schematic illustration of the lung metastatic model in nude mice with negative control or circXRN2-overexpressing cells. k-l Overexpressing circXRN2 led to fewer lung metastatic nodules than in the control group. All the data are presented as the mean ± standard deviation (n = 5). *P < 0.05, **P < 0.01, compared with the control group