Figure 4.

Venn diagrams for the differentially expressed genes in the cortex. (A) Venn diagram for the DEGs in the cortex of 5XFAD (females and males, n = 3 + 3), MCAO-24h (females, n = 3), TBI-7d (females, n = 3), and Prions (females and males, n = 3 + 3). Blue numbers show the portion of DEGs that are common with the Aged group (females and males, n = 3 + 3). (B) Venn diagrams representing overlaps in DEGs detected for Aged cortex and for cortices of other groups. (C) Venn diagrams of the DEG percentage overlap in the reporters of astrocyte reactivity (left) and function (right), presented for the groups with strong changes in gene expression (top) and mild changes in gene expression (bottom). Genes annotated as A1, A2, pan-reactive, other markers of reactive astrocytes, regulators of astrocyte reactivity, and astrocyte-specific markers were considered as reporters of reactivity. Genes related to BBB regulation, lipid and energy metabolism, extracellular matrix, junction, myelination, channels, transporters, gliotransmitters and neurotransmitters, and neuroprotection were included into Function DEGs. Differential expression analysis was performed using NanoString Advanced Analysis software 2.0.115. Each group was compared to their corresponding controls (Table 1). DEGs for the Aged group were calculated relative to the youngest control group (TBI-7d Ctrl, n = 3F). The genes with linear fold greater than 1.2 up or down and adjusted p < 0.1 were selected as DEGs (Table S3). Ctx refers to the cortex. TBI-7d is represented by the ipsilateral Ctx.