Figure 2.

Procedure and characteristics of an orthotopic model of gastric cancer. Yellow cycles highlight the focus of the photos. (A) The illustration depicts the orthotopic injection of tumor cells into the stomach wall. (B) Representative bioluminescence images of injected mice. (C) Representative MRI images of injected mice taken 3 weeks after injection. (D) Representative macroscopic images of the abdominal cavity and peritoneal membrane of injected mice, as well as resected stomach and the developed primary tumor (arrow). (E) Representative hematoxylin and eosin (H&E)-stained images of tumoral tissues in multiple structures. (F) Survival curve of orthotopic mice injected with 0.1 million (n = 21) or 1 million (n = 12) tumor cells. (G) Graphical illustration of the orthotopic gastric cancer model and its progression into various organs.