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Nested PCR of E. risticii for the 16S rRNA (527 bp), groESL heat shock operon (526 bp), and 51-kDa major antigen (569 bp) genes with DNA from Juga secretions (lanes 1), concentrated tank water (lanes 2), and positive (lanes +) and negative (lanes −) E. risticii DNA controls. φ, φX174 replicative-form DNA HaeIII digest (molecular size marker).
