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. 2023 Sep 4;12(17):2205. doi: 10.3390/cells12172205

Figure 3.

Figure 3

F-actin and FN1 at different flow rates in the 2D model. (A): Confocal image z-stack projections of F-actin and FN1 in HTFs exposed to different flow rates or TGF-β1 (5 ng/mL) are displayed. Cells were preincubated in µ-slides for 24 h in starvation medium (0.2% FBS), then perfused for 72 h with the respective flow rate or stimulated with TGF-β1 (5 ng/mL) in starvation medium (0.2% FBS). Image acquisition and representation settings are identical for all conditions. The figure is representative of three independent experiments. (B): The relative change (RC) in mean signal intensities for F-actin and FN1 in the projected confocal image stacks of three independent experiments, as displayed in Figure 3A, is shown. Image acquisition and representation are identical under different conditions. Asterisks indicate levels of significance in Dunnett’s t-test (* p < 0.05, ** p < 0.01, *** p < 0.001).