Figure 4.

Tz/T-DM1 plus Lp inhibit cell adhesion and migration. HER2+ BC cells were treated with Tz (1 μg/mL), T-DM1 (1 μg/mL), Lp (0.1 µg/mL) or their combinations for 72 h, and cell adhesion and migration assays were performed. (A–C) Representative images and percentages of cells adhered to gelatin, and (D,E) representative images and percentages of SKBR3 cell migration. Gap closure was quantified by measuring areas using the ImageJ software. Adhesion/migration results are expressed as a percentage of attached/migrated cells vs. control cells. * = p < 0.05 vs. control, • = p < 0.05 in combined treatments vs. Tz/T-DM1, and ° = p < 0.05 in combined treatments vs. Lp. All experiments were performed in triplicate; representative images are shown.