Table 3.
Examples of the reduction in melting temperature that can be achieved in mRT-PCR using 3base™ technology.
| 4Base Sequence | 3Base™ Sequence | |||
|---|---|---|---|---|
| Target | Sequence | Tm °C | Sequence | Tm °C |
| Primer-1 | GTACACACCGCCCGTCGCTCCTACC | 66.6 | GTATATATTGTTTGTTGTTTTTATT | 51 |
| Primer-2 | GAGGAAGGTGAAGTCGTAACAAG | 55.2 | GAGGAAGGTGAAGTTGTAATAAG | 49.7 |
| Entamoeba spp. Probe | TGAATAAAGAGGTGAAATTCTAGG | 51.3 | TGAATAAGAGGTGAAATTTTAGG | 49 |
| G. intestinalis Probe | GCGCGAAGGGCCGCGAGCCCCCGCGC | 78.8 | GTGTGAAGGGTTGTGAGTTTTTGTGT | 57.9 |
| D. fragilis Probe | TTTAAATGAAAAGGTGATTAAATCACG | 51.2 | TTTAAATGAAAAGGTGATTAAATTATG | 47.7 |
| Cryptosporidium spp. Probe | GACCATACTTTGTAGCAATACATGTAAGGA | 56.6 | GATTATATTTTGTAGTAATATATGTAAGGA | 48.1 |
| B. hominis Probe | TATTGAAAGAAGTTGTGTAAATCTTACCATT | 53.9 | TATTGAAAGAAGTTGTGTAAATTTTATTATT | 50 |