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The upregulation of miR-616 during ER stress is dependent on the PERK pathway. MCF7 PLKO, MCF7 XBP1-KD, MCF7 PERK-KD and MCF7 ATF6-KD sub-clones were treated with (0.5 µg/mL) BFA for 24 h. The expression level of (A) CHOP, (B) pri-miR-616 was quantified by RT-qPCR, normalizing against RPLP0. (C,D) The expression level of miR-616-5p and -3p was quantified by RT-qPCR, normalized using RNU6. * p < 0.05, ** p < 0.001, two-tailed unpaired t-test. ns, not significant.
