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. 2023 Aug 25;24(17):13222. doi: 10.3390/ijms241713222

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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mRNA expression of IL-32 and CCL20 in liver samples from patients with MASLD. (A) IL-32 and (B) CCL20 mRNA expression was analyzed in liver tissue samples from patients with MASH (SH, n = 43), hepatic steatosis (S, n = 46) and normal liver tissue (N, n = 32) by qRT-PCR. Expression levels were plotted regarding their histologically proven steatosis grade (0 = 32, 1 = 25, 2 = 44, 3 = 21) and fibrosis grade (0 = 76, 1–2 = 27, 3–4 = 16) (for sample characteristics see Table S1). YWHAZ mRNA expression was determined for normalization. Statistical differences were analyzed by Kruskal–Wallis test with post hoc Bonferroni correction. * p < 0.05, ** p < 0.01.