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. 2023 Aug 25;24(17):13222. doi: 10.3390/ijms241713222

Figure 3.

Figure 3

Unsaturated oleic acid (OA) reduces PA-induced expression of IL-32 and CCL20 in vitro. (A) HepG2 cells were incubated either with 0.4 mM PA or with 0.4 mM PA/OA (1/2) for indicated times (control, no treatment). The mRNA levels were analyzed and normalized to YWHAZ (three independent experiments, mean ± SD). * p < 0.05 differs from control. (B) HepG2 cells were incubated without PA, with 0.4 mM PA or 0.4 mM PA/OA (1/2) for 24 h followed by isolation of total protein extracts. Western blot analysis using specific anti-IL-32 and anti-CCL20 antibodies was performed with β-actin as loading control. Immunoblots were analyzed by densitometry and normalized to loading control and untreated cells (control). Data presented as mean ± SEM, * p < 0.05 differs from control, # p < 0.05 differs from PA treatment.