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. 2023 Aug 25;24(17):13222. doi: 10.3390/ijms241713222

Figure 4.

Figure 4

PA-induced expression of IL-32 and CCL20 is mediated by Erk1/2 and p38 MAPK. (A) Huh7 cells were treated with 0.4 mM PA for 24 h, and without (no inhibition) or with (1.5 h prior to PA) addition of specific inhibitors: PD098059 (PD, 10 µM) for Erk1/2, SB203580 (SB, 10 µM) for p38 MAPK or GF109203X (GX, 10 µM) for PKC signaling. The mRNA levels were analyzed and normalized to YWHAZ (three independent experiments, mean ± SD). * p < 0.05 differs from control, # p < 0.05 differs from no inhibition. (B) Huh7 and HepG2 cells were treated without or with 0.4 mM PA for 24 h, and with or without PD, SB or GX as described above (A) followed by isolation of total protein extracts. Western blot analysis using specific anti-IL-32 and anti-CCL20 antibodies was performed with β-actin as loading control. Immunoblots were analyzed by densitometry and normalized to loading control and untreated cells (control). Data presented as mean ± SEM, * p < 0.05 differs from control, # p < 0.05 differs from no inhibition.