TABLE 3.
Agreement of results of sodium chloride tolerance test for rapidly growing Mycobacteria with expected results for all organisms tested at 35°C in ambient air with various inoculum concentrationsa
Organism | Expected result | No. of organisms/no. of tests | Suspension Ab
|
Suspension Bc
|
Suspension Cd
|
||||||
---|---|---|---|---|---|---|---|---|---|---|---|
No. of tests pos | No. of tests neg | % Correct | No. of tests pos | No. of tests neg | % Correct | No. of tests pos | No. of tests neg | % Correct | |||
M. fortuitum | Pos | 6/18 | 18 | 0 | 100 | 18 | 0 | 100 | 18 | 0 | 100 |
M. peregrinum | Pos | 4/12 | 12 | 0 | 100 | 11 | 1 | 92 | 9 | 3 | 75 |
M. chelonae | Neg | 4/12 | 0 | 12 | 100 | 0 | 12 | 100 | 0 | 12 | 100 |
M. abscessuse | Pos | 6/18 | 14 | 4 | 78 | 13 | 5 | 72 | 8 | 7 | 44f |
M. abscessus variant | Pos | 5/15 | 15 | 0 | 100 | 15 | 0 | 100 | 10 | 2 | 67g |
M. mucogenicum | Neg | 6/18 | 0 | 18 | 100 | 0 | 18 | 100 | 0 | 18 | 100 |
Expected results are as defined by Kent and Kubica (3). LJ Salt from three manufacturers, BBL, Difco, and Remel, were used. pos, positive; neg, negative.
A 1 McFarland standard suspension.
A barely turbid suspension equal to 86 ± 2%T at 530 nm.
A 1:2,500 dilution of a 1 McFarland standard suspension.
Includes a salt tolerance test-negative M. abscessus isolate.
Results for 3 of 18 tests were uninterpretable.
Results for 3 of 15 tests were uninterpretable.