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. 2023 Sep 5;35(9):1630–1645.e5. doi: 10.1016/j.cmet.2023.07.003

Figure 2.

Figure 2

Neddylation is required for the gluconeogenic action of counter-regulatory hormones

(A–D) Blood glucose after intraperitoneal (i.p.) administration of saline or glucagon (200 μg kg−1) for (A) WT mice treated with vehicle or MLN4924, (B) NAE1+/− mice or their control littermates, (C) Alfp-Cre+/− mice injected with AAV-DIO expressing either GFP or shNAE1 and (D) Alfp-Cre+/− mice injected with AAV-DIO expressing either GFP or shNEDD8.

(E–H) Blood glucose levels after i.p. administration of saline or adrenaline (100 μg kg−1) for (E) WT mice treated with vehicle or MLN4924, (F) NAE1+/− and their control littermates, (G) Alfp-Cre+/− mice injected with AAV-DIO expressing either GFP or shNAE1 and (H) Alfp-Cre+/− mice injected with AAV-DIO expressing either GFP or shNEDD8.

(I–L) Blood glucose after i.p. administration of saline or hydrocortisone (20 mg kg−1) for (I) WT mice treated with vehicle or MLN4924, (J) NAE1+/− mice or their control littermates, (K) Alfp-Cre+/− mice injected with AAV-DIO expressing either GFP or shNAE1 and (L) Alfp-Cre+/− mice injected with AAV-DIO expressing either GFP or shNEDD8; n = 3–10 per group. Data are presented as mean ± SEM; one-way ANOVA followed by Bonferroni post hoc test: ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001. # denotes differences among the different animal groups treated with the same glucoregulatory hormone.