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Transcription factor SbbHLH041 binds to SbEXPA11-promoter. (a) Yeast one-hybrid (Y1H) analysis of the binding of SbbHLH041 to SbEXPA11-promoter (SbEXPA11prom). (b) EMSA showing the binding of SbbHLH041 to the −1972~−1945 region of SbEXPA11prom. (c) Y1H analysis of the binding of SbbHLH041 to the −1972~−1945 region of SbEXPA11prom. (d) EMSA showing the binding of SbbHLH041 to the E-box (bold) of SbEXPA11prom at −1972~−1945 region. Mutated bases are underlined.
