Figure 6.

Effects of psoralen on UUO-induced renal fibrosis. Expression levels of TGF-β1, pSmad2/3, and α-SMA (A) were analyzed by immunoblotting. Quantitative analyses of TGF-β1, pSmad2/3, and α-SMA were performed. Results were normalized to levels of β-actin (B). α-SMA immunohistochemical staining was performed to verify fibrosis (C). mRNA expression levels of TGF-β1 and Snail were analyzed by quantitative real-time PCR (D). Scale bar, 50 μm. Data are presented as means ± SEMs. * p < 0.05. Sham: no ureteral ligation; PS: only PS treatment; UUO: no PS treatment, but ureteral ligation; UUO + PS: PS treatment and ureteral ligation group.