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. 2023 Aug 31;24(17):13536. doi: 10.3390/ijms241713536

Figure 6.

Figure 6

ECM remodeling is different in Cre+ versus Cre− mice as reflected in the changes in laminin expression. Green color represents Cre− data and red color refers to Cre+ data. Numbers in brackets indicate the number of muscle samples. (A) Relative laminin mRNA expression (normalized to AP3D1 as the internal control) in m. tibialis anterior from young Cre− and Cre+ mice 4 days after BaCl2 injection for control (C) and injected (I) legs. (B) Relative laminin protein expression (normalized to α-actinin as the internal control) in m. tibialis anterior from young Cre− and Cre+ mice 4 days after injection. (C) Representative images of Western blot showing expression changes in laminin protein on day 4. (D) Relative laminin mRNA expression in m. tibialis anterior from young Cre− and Cre+ mice 14 days after injection. (E) Relative laminin protein expression in m. tibialis anterior from young Cre− and Cre+ mice 14 days after injection. (F) Representative images of Western blot showing expression changes in laminin protein on day 14. Asterisks indicate statistically significant difference (* p < 0.05, *** p < 0.001 Student’s t-test). Actual p values when p > 0.001 are: (A) Cre− C vs. Cre− I p = 0.002; Cre+ C vs. Cre+ I p = 0.006, Cre+ C vs. Cre+ I p = 0.874; (B) Cre− C vs. Cre− I p = 0.039; Cre+ C vs. Cre+ I p = 0.46; (D) Cre− C vs. Cre− I p = 0.126; (E) Cre− C vs. Cre− I p = 0.92; Cre+ C vs. Cre+ I p = 0.90.