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Illustration of SOD1 and WT NMJ culture with and without creatine treatment. (A) Cartoon illustration of the NMJ chamber. (B) Experimental timeline for iPSC hSKM and hMN plating and subsequent creatine treatment. (C) Phase images for the co-cultures of hSKMWT hMNWT, hSKMSOD1 hMNWT and hSKMSOD1 hMNSOD1 with/without creatine. Scale bar = 100 µm.
