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. 2023 Aug 31;24(17):13519. doi: 10.3390/ijms241713519

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Quantification of the fatigue index for NMJ-stimulated SKM and direct SKM stimulation in the D14 NMJ co-culture with and without creatine treatment. (A) NMJ fatigue index collected under MN stimulation. (B) SKM fatigue index collected under SKM direct stimulation. Results from the NMJ co-cultures of different genetic combinations are displayed: (A,B) In the co-culture of hSKM_SOD1 hMN_WT, fatigue index of NMJ (A) and SKM (B). (C,D) In the co-culture of hSKM_SOD1 hMN_SOD1, fatigue index of NMJ (C) and SKM (D). (i) WT (ii) SOD1 E100G (iii) SOD1 L144P. Data: Mean. Error bars: SEM * p < 0.1, ** p < 0.01, *** p < 0.001.