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Immunocytochemistry analysis of the D17 NMJ co-culture with and without creatine treatment utilizing the markers BTX-488 (green) for AchR and Myosin Heavy Chain (MyHC) (red) or Synaptophysin (syn, red) for myotubes. (A) For the co-cultures containing the SOD1 E100G mutation. (B) For the co-cultures containing the SOD1 L144P mutation. Sample images from the NMJ co-cultures of different genetic combinations are displayed: hSKMSOD1 hMNWT and hSKMSOD1 hMNSOD1. Scale bar = 100 µm.
