Table 1.
Preliminary preparation of CLEA-LCs using 5 mg of free CAL-B in 10 mL of 25 mM sodium phosphate buffer pH 7.0. Immobilization yield (I.Y, (%)), specific activity (S.A, U/mg) and activity yield (A.Y, %) are described in Section 4.2.4.
| Group | Case | Molar Equivalents. CAL-B.: GA: PTR. |
V GA./V% (µL) |
V PTR.
(µL) |
I.Y. (%) |
S.A. CLEAs (U/mg) | A.Y. (%) |
|---|---|---|---|---|---|---|---|
| G1 | 01 | 1: 250: 250 | 15/0.15 | 110 | 10 | 35 | 100 |
| G2 | 02 | 1: 500: 250 | 30/0.30 | 110 | 13 | 35 | 100 |
| 03 | 1: 500: 500 | 30/0.30 | 220 | 15 | 34 | 96 | |
| 04 | 1: 500: 1000 | 30/0.30 | 440 | 18 | 34 | 97 | |
| G3 | 05 | 1: 1000: 250 | 60/0.60 | 110 | 15 | 35 | 100 |
| 06 | 1: 1000: 500 | 60/0.60 | 220 | 19 | 35 | 100 | |
| 07 | 1: 1000: 1000 | 60/0.60 | 440 | 25 | 36 | 103 | |
| G4 | 08 | 1: 2000: 250 | 120/1.18 | 110 | 20 | 34 | 97 |
| 09 | 1: 2000: 500 | 120/1.18 | 220 | 23 | 35 | 100 | |
| 10 | 1: 2000: 1000 | 120/1.18 | 440 | 35 | 37 | 106 | |
| 11 | 1: 2000: 2000 | 120/1.18 | 880 | 29 | 30 | 86 |