Figure 2.

A comparison of the global primary AML cell proteome for leukemic cells showing strong antiproliferative effects of bafilomycin A1 10 nM (eight patients, relative responses ≤ 0.30) and weak/no antiproliferative effects of bafilomycin (seven patients, relative responses ≥ 0.60). (A) Unsupervised hierarchical clustering analysis that included all 15 patients and, based on the expression of 61 differentially expressed proteins, identified two main patient clusters. The clustering of the differentially expressed proteins is presented in the left part of the figure, and the proteins are listed from the top of the clustering analysis and downwards in Table S6. (B) Gene ontology (GO) analyses of differentially expressed proteins showing significantly increased levels in AML cells characterized by strong antiproliferative effects of bafilomycin A1 10 nM (upper part) and cells characterized by weak/no antiproliferative effects (lower part). These analyses were based on identification of biological processes (BP), molecular functions (MF), cell compartment (CC) and KEGG classification, as indicated at the top of the figure (* Significant GO terms and KEGG pathway with unadjusted p-value).