Figure 3.

A comparison of the global primary AML cell phosphoproteome for leukemic cells characterized by either a strong antiproliferative effect of bafilomycin A1 10 nM (eight patients, relative response ≤ 0.30) or a weak effect (seven patients, ≥0.60). (A) Unsupervised hierarchical clustering analysis including all 15 patients and based on all 100 differentially expressed phosphorylation sites identified two main patient clusters. The proteins are listed from the top of the heatmap and downwards in Table S9. (B) The figure presents the results from a volcano plot analysis based on all differentially expressed phosphorylation sites, and the indicated points above the non-axial horizontal grey line represent protein sites with significantly different phosphorylation (p < 0.05). (C) Protein–protein interaction (PPI) analyses of differentially phosphorylated proteins.