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. 2023 Aug 29;24(17):13397. doi: 10.3390/ijms241713397

Figure 4.

Figure 4

Effect of aging and MDL72527 on DNA damage. (A) Cells were cultured in the absence (none) or presence (MDL) of 20 µM MDL72527 for 3 days (young) and serially passaged for 3 months (aged). Cells were seeded on coverslips, cultured for 1 d, and fixed. DNA damage was visualized as described in the Materials and Methods section. Red fluorescence indicates phospho-γH2AX, and blue fluorescence indicates nuclei. Bar = 20 μm. (B) Red fluorescence intensity in cells was measured as described in the Materials and Methods section and shown as mean + SD of at least 10 cells from three independent staining. Open columns and filled columns indicate none and MDL, respectively. ** p < 0.01, *** p < 0.005.