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. 2023 Aug 30;24(17):13490. doi: 10.3390/ijms241713490

Figure 2.

Figure 2

The frequency of GABAergic miniature PSCs in hippocampal NG2 cells is lower in adult animals. (A) Original recordings of mPSCs in NG2 cells of juvenile (upper trace) and adult (lower trace) animals. For recording, 0.5 µM TTX was added to the blocking solution (10 µM NBQX, 50 µM D-APV and 5 µM CGP55845). Gray diamonds indicate events. In p60 mice only few mPSCs could be observed. Scale bar shows 10 pA and 1 s. (B) Boxplots showing interevent interval and frequency of mPSCs for all recorded cells. At age p60, the interevent interval was significantly higher (p60: 54.02 s, p10: 15.28 s, p < 0.01) and the frequency was significantly lower (p60: 0.0191 Hz, p10: 0.063 Hz, p < 0.01) than at age p10. (C) Recorded mPSCs (gray lines) and their average (red line) for a single NG2 cell at ages p10 (upper trace) and p60 (lower trace). Scale bar shows 5 pA and 20 ms. (D) Boxplots showing peak amplitude (p10: −7.54 pA; p60:–7.46 pA, p = 0.76), rise time (p10: 2.01 ms; p60: 2.27 ms; p = 0.59) and decay time of mPSCs (p10: 17.16 ms; p60: 16.73 ms; p = 0.39) did not differ between juvenile and adult mice. Mann–Whitney-U test, ** p < 0.01. p10: n = 20, N = 9; p60: n = 10, N = 9.