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. 2023 Aug 30;24(17):13490. doi: 10.3390/ijms241713490

Figure 7.

Figure 7

Hippocampal NG2 glia express transcripts for postsynaptic and extrasynaptic GABAA receptor clustering proteins and auxiliary receptor subunits. (A,B) Single-cell RT-PCR of NG2 glia was performed to detect GABAA receptor anchoring proteins. Current patterns (voltage steps between −160 mV and +20 mV, 10 mV increment, holding potential −80 mV) are shown together with the respective agarose gel of PCR products for collybistin (CBS), gephyrin (Geph), radixin (Rdx) and GARLH4 (LHF4) at p10 (A) and p60 (B). PDGFRα served as positive control. (C) Single-cell RT-PCR analysis of different GABAA receptor associated anchoring proteins at p10 (0.86, n = 21; 0.85, n = 20; 0.71, n = 21; 0.5, n = 18) and p60 (0.82, n = 22; 0.69, n = 16; 0.68, n = 22; 0.76, n = 17). There were no age-dependent differences (p > 0.1 in all cases, Chi-square test). (D) Real-time RT-PCR analysis for radixin performed on FAC-sorted NG2 glia from the hippocampus at p10 (normalized values; 0.08 ± 0.04, n = 15) and p60 (0.068 ± 0.02, n = 13). No age-dependent differences were found (t-test, p = 0.33).