Table 1.
Primers used for single-cell RT-PCR.
| Gene | Primer Sequence | Product Length | Position | Genbank Accession Number |
|---|---|---|---|---|
| Collybistin (Arhgef9) | se 5′-CACGGAGCGCCATTACATCAA as 5′-GGCGGCAGGCCTCAAAGA |
338 bp | 339 659 |
NM_001033329 |
| Collybistin (Arhgef9) (nested) | se 5′-GTGCCGAAAGAGAAGGGACAT as 5′-CAGGCATCCAGGTGGTTGTTA |
219 bp | 396 594 |
|
| Gephyrin | se 5′-AGGTGCAGCAGCAAGGAGAACATT as 5′-GTTGTAACCCGCATCACTTGTCCT |
365 bp | 982 1323 |
NM_145965 |
|
Gephyrin (nested) |
se 5′-TCTCCTTTTCCCCTGACG as 5′-TCCCCAATGATGAAACGAT |
221 bp | 1063 1265 |
|
| Radixin | se 5′-CAGCGAGCAAAAGAGGAGGCAGAG as 5′-TGCTTCTTCACGCGTTCATTCTTC |
443 bp | 1138 1557 |
NM_009041 |
|
Radixin (nested) |
se 5′-GCAAGCTGCTGACCAGATGAAG as 5′-TCCGTGGGAGGGATGACTG |
234 bp | 1215 1430 |
|
| LHFPL4 | se 5′-TCTACAAGATCTGCGCCTGGATGC as 5′-TGGCCGCAGCACGGAACTTACTGT |
311 bp | 371 658 |
NM_177763 |
|
LHFPL4 (nested) |
se 5′-ATGGCTGGGATGCTGAGACC as 5′-ACTGTAGTGCCCACGAAATCTTTG |
220 bp | 443 639 |
|
| GABAA-R α3 (Gabra3) | se 5′-TAACCGGCTTCGACCTGGACTTG as 5′-CAGCGTGTATTGTTAACCTCATTG |
332 bp | 237 545 |
NM_008067 |
| GABAA-R α3 (Gabra3) (nested) |
se 5′-AGTTTTGGCCCTGTGTCAGAC as 5′-AGAGGAGGGTCCCATTATCTACC |
241 bp | 301 519 |
Position 1 is the first nucleotide of the initiation codon. The length of PCR products was indicated as base pairs (bp). ‘se’ and ‘as’ indicate sense and antisense primers. All sense and antisense primers are located on different exons, respectively.