Figure 6.
(a) Chemical structures of fluorescent probe 38 and sensing mechanisms to detect esterase activity and cell viability. (b) Fluorescent imaging of TPC1 cells stained with 38 at different incubation times ((A,F,K) 5 min; (B,G,L) 15 min; (C,H,M) 30 min; (D,I,N) 45 min; (E,J,O) 60 min) in different fluorescent channels ((A–E) yellow channel (525–585 nm); (F,J) red channel (625–685 nm) and (K–O) merge image). λex = 490 nm. Scale bar = 10 μm [34,39]. Reprinted with permission from [39] Copyright © 2018 American Chemical Society.