Figure 4.

(a) In vivo TPM images of Aβ plaques in the frontal cortex of a 5XFAD mouse after ip injection of DN (10 mg kg⁻¹), 20× magnified at the depth of 50 μm (scale bar: 20 μm). (b) Magnified images (60×) of the square area in (a) (scale bar: 10 μm). (c) 3D images: the images were acquired with 20× magnification along the z-direction at the depth of up to 300 μm from the surface of the cortex, under excitation at 1000 nm with approximately 50 mW laser power at the focal point. Reproduced with permission from Ref. [74]. Copyright 2015 American Chemical Society.