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. 2023 Aug 23;28(17):6201. doi: 10.3390/molecules28176201

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) SdFFF sorting scheme of glioblastoma cell lines: F1 = subpopulations with specific differentiation characteristics; F2 = cancer stem cells (CSCs) and high-frequency crossover (HFC) values. (b) Functional characterization of F1, F2, total peak collected (TP), and unfractionated cells (crude). *** p < 0.0001 (ANOVA). (A) Cell cycle analysis by DNA content measurement: CSCs (F2) are found in the G1 phase (quiescent cells). (B) Soft agar assay for colony formation examination: the F2 subpopulation consists of a population of cells enriched in CSCs, whereas the F1 subpopulation is enriched in differentiated cells. * p < 0.05; ** p < 0.001 (Student’s t test). Images adapted from [242].